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Isolatio of yeast in the RNA, Study notes of Biochemistry

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Joise Daria M. Alabo BSMSLS 2-A-7
CHEM 113 Nucleic Acid
Watch the supplemental video - chemical tests for the components of nucleic acids
before answering this activity.
Input your observations in a document based on the table below. Attach your file here.
1- Solubility of Nucleic Acid Extracted from Yeast
SOLVENT
SOLUBILITY OF NUCLEIC ACIDS
Cold water
Insoluble
Hot water
Slightly soluble
Ethanol
Insoluble
Diluted HCl
Insoluble
Diluted NaOH
Very Soluble
2- Qualitative Tests for Nucleic Acids
Name of Test
Observation
Explanation
Biuret Test for
Nucleoproteins (filtrate from
yeast RNA)
When NaOH solution and
CuSO4 solution is added in
a 1 mL of the filtrate
(isolation of yeast), the color
change. The test tube that
has NaOH solution formed
a purple color while the
solution with CuSO4 formed
a blue color
Nucleoproteins formed a
purple color in a solution
which indicate that the
solution containing NaOH
and 1mL of the filtrate has a
nucleoprotein, because it
gives positive result.
a. Test for phosphates
(acid hydrolysate
When HNO3 and
Ammonium Molybdate is
added to the 1 mL of the
filtrate treated mild acid
hydrolysis the color change
Because it formed a yellow
precipitate which indicates
the presence of inorganic
phosphate in the solution
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Joise Daria M. Alabo BSMSLS 2 - A- 7 CHEM 113 Nucleic Acid Watch the supplemental video - chemical tests for the components of nucleic acids before answering this activity. Input your observations in a document based on the table below. Attach your file here. 1 - Solubility of Nucleic Acid Extracted from Yeast SOLVENT SOLUBILITY OF NUCLEIC ACIDS Cold water Insoluble Hot water Slightly soluble Ethanol Insoluble Diluted HCl Insoluble Diluted NaOH Very Soluble 2 - Qualitative Tests for Nucleic Acids Name of Test Observation Explanation Biuret Test for Nucleoproteins (filtrate from yeast RNA) When NaOH solution and CuSO4 solution is added in a 1 mL of the filtrate (isolation of yeast), the color change. The test tube that has NaOH solution formed a purple color while the solution with CuSO4 formed a blue color Nucleoproteins formed a purple color in a solution which indicate that the solution containing NaOH and 1mL of the filtrate has a nucleoprotein, because it gives positive result. a. Test for phosphates (acid hydrolysate When HNO3 and Ammonium Molybdate is added to the 1 mL of the filtrate treated mild acid hydrolysis the color change Because it formed a yellow precipitate which indicates the presence of inorganic phosphate in the solution

and it formed a yellow precipitate b. Test for sugar 0.1% ribose Formed a blue-green complex The formation of blue green complex in 10% ribose solution indicates that the solution is pentose 0.1% glucose Does not form a blue green complex Glucose does not form a blue-green complex because the 10% glucose solution is not a pentose. Glucose is an hexose a monosaccharide with six carbon atoms. Acid hydrolysate Formed a blue-green complex The formation of blue green complex in Acid hydrolysate indicates that the solution also have a pentose because the RNA in the solution. c. Test for purine bases (acid hydrolysate) Formed a white precipitate The formation of white precipitate in the in solution indicates a positive result which means that it has a presences of purines in the solution.