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Genetics - Introduction, Lecture notes of Genetics

This document is about the basic terms in Genetics.

Typology: Lecture notes

2022/2023

Available from 07/01/2024

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Genetics
LESSON 1
INTRODUCTION TO GENETICS
The Work of Gregor Mendel
Every living thing - plant or animal,
microbe, or human being - has a set of
characteristics inherited from its
parents.
Since the beginning of recorded
history, people have wanted to
understand how that inheritance is
passed from generation to generation.
Genetics the scientific study of heredity
and variation.
Gregor Mendel
Austrian Monk
Born 1822 in the Czech Republic
Worked at a monastery and taught
high school.
Tended the monastery garden.
Grew peas and became interested in
the traits that were expressed in
different generations of peas.
True breeding
If allowed to self-pollinate they would
produce offspring identical to
themselves
He was also able to crossbreed peas
for different traits.
Genes and Dominance
Mendel studied seven different pea
plant traits.
Each trait he studied had a contrasting
form.
The offspring of crosses between
parents with different traits are called
Hybrids.
When Mendel crossed plants with
different traits, he expected them to
blend, but that's not what happened at
all.
All of the offspring had the character of
only one of the parents.
Mendel drew two conclusions:
1. Inheritance is determined by
factors that are passed from
generation to generation - today
we call these factors genes.
2. The Principal of Dominance
Some alleles are dominant, and
some are recessive.
Alleles – different forms of genes
Dominant – cover up the recessive from
Ex: T = tall
Recessive gets covered up with the
presence of a dominant allele.
Ex: t = short
Segregation
Mendel wanted to answer another
question.
Q: Had the recessive alleles disappeared?
Or where present in the F1 plants?
To answer this he allowed the F, plants
to produce an F 1 generation by self-
pollination.
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Genetics

LESSON 1

INTRODUCTION TO GENETICS

The Work of Gregor Mendel

  • Every living thing - plant or animal, microbe, or human being - has a set of characteristics inherited from its parents.
  • Since the beginning of recorded history, people have wanted to understand how that inheritance is passed from generation to generation. Genetics – the scientific study of heredity and variation. Gregor Mendel
  • Austrian Monk
  • Born 1822 in the Czech Republic
  • Worked at a monastery and taught high school.
  • Tended the monastery garden.
  • Grew peas and became interested in the traits that were expressed in different generations of peas. True breeding
  • If allowed to self-pollinate they would produce offspring identical to themselves
  • He was also able to crossbreed peas for different traits. Genes and Dominance
  • Mendel studied seven different pea plant traits.
  • Each trait he studied had a contrasting form. - The offspring of crosses between parents with different traits are called Hybrids. - When Mendel crossed plants with different traits, he expected them to blend, but that's not what happened at all. - All of the offspring had the character of only one of the parents. Mendel drew two conclusions: 1. Inheritance is determined by factors that are passed from generation to generation - today we call these factors genes. 2. The Principal of Dominance - Some alleles are dominant, and some are recessive. Alleles – different forms of genes Dominant – cover up the recessive from Ex: T = tall Recessive – gets covered up with the presence of a dominant allele. Ex: t = short Segregation - Mendel wanted to answer another question. Q: Had the recessive alleles disappeared? Or where present in the F 1 plants? - To answer this he allowed the F, plants to produce an F 1 generation by self- pollination.

The F 1 Cross

  • The recessive traits reappeared!
  • Roughly 1/4 of the F 1 plants showed a recessive trait. Explanation of the F 1 Cross
  • The reappearance indicated that at some point the allele for shortness had been separated from the allele for tallness.
  • Mendel suggested that the alleles for tallness and shortness in the F 1 plants were segregated from each other during the formation of sex cells or gametes.
  • When each F 1 plant flowers, the two alleles segregate from each other so that each gamete carries only a single copy of each gene. Therefore, each F 1 plant produces two types of gametes - those with the allele for tallness and those with the allele for shortness. Probability and Punnett Squares Mendel kept obtaining similar results, he soon realized that the principals of probability could be used to explain the results of genetic crosses. Probability
  • The likelihood that a particular event will occur.
  • The way in which alleles segregate is random like a coin flip. Punnett Square - Diagram used to determine genetic crosses. Homozygous - Organisms that have 2 identical alleles for a trait. Ex.) TT, tt Heterozygous - Have two different alleles for a trait. Ex.) Tt Phenotype - Physical characteristics (words) Ex.) tall Genotype - Genetic make-up (letters) Ex.) Tt, TT, t Exploring Mendelian Genetics Independent Assortment - Genes that segregate independently do not influence each other’s inheritance. Incomplete dominance
    • When one allele is not dominant over another
    • Four o'clock flowers
    • The heterozygous phenotype is somewhat in between the two homozygous phenotypes.

Each chromosome pairs with its corresponding homologous chromosome to form a structure called a Tetrad - has 4 chromatids. Crossing over - When chromosomes exchange portions of their chromatids and results in the exchange of alleles

  • Leads to new combinations of alleles.
  • The homologous chromosomes separate, and 2 new cells are formed.
  • Although each cell now has 4 chromatids something is different. Because each pair of homologous chromosomes was separated, neither of the daughter cells has two complete sets of chromosomes that it would have in a diploid cell.
  • The two sets have been shuffled. Meiosis II
  • The two cells produced by meiosis I now enter a second meiotic division.
  • Unlike the 1st division, no chromosomes are replicated.
  • Each cell's chromosomes have 2 chromatids. Metaphase II
  • 2 chromosomes line up in the center of each cell Anaphase Il
  • The paired chromatids separate Telophase II
  • Forms 4 daughter cells each with 2 chromatids
  • These 4 daughter cells are now haploid (N) - just 2 chromosomes each Gamete Formation
  • In male animals, the haploid gametes produced by meiosis are called sperm.
  • In some plants, they are called pollen.
    • In females, generally only one of the cells produced by meiosis is involved in reproduction.
    • This female gamete is called an egg.
    • The other 3 cells that do not receive as much cytoplasm as the egg are called polar bodies Comparing Mitosis and Meiosis
    • Mitosis results in the production of two genetically identical diploid cells, whereas meiosis produces four genetically different haploid cells. Linkage and Gene Maps Gene Linkage
    • When genes are located on the same chromosome they are inherited together (Linkage)
    • It's the chromosomes that assort independently not individual genes.
    • When genes are formed on the same chromosome, this does not mean that they are linked forever.
    • Crossing over during meiosis sometimes separates genes that had been on the same chromosome onto homologous chromosomes.
  • Cross-over events occasionally separate and exchange linked genes and produce new combinations of alleles. Gene Maps
  • 1911 Alfred Sturtevant
  • hypothesized that the further apart genes were, the more likely they were to be separated by a crossover in meiosis.
  • the rate at which linked genes were separated and recombined could then be used to produce a " map " of distances between genes.
  • Shows the location of each gene.