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DNA Fingerprinting - Forensic Science - Lecture Notes, Study notes of Forensics

Forensic science (often shortened to forensics) is the application of a broad spectrum of sciences and technologies to investigate and establish facts of interest in relation to criminal or civil law. This lecture includes: DNA Fingerprinting, Scientists, Fingerprinting, Regular Cell, Chromosomes, Sex Cells, Chromosomes, Sugar, Deoxyribose, Nitrogen Base

Typology: Study notes

2012/2013

Uploaded on 02/01/2013

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DNA Fingerprinting
Scientists first discovered DNA in the 1800’s but it had no interest for the legal system until 1985
when___________________________ revealed his research that suggested that DNA is a unique as a
fingerprinting. (thus the term DNA fingerprinting)
The Basics
We have about 60 trillion cells and each of those cells contains a full set of our chromosomes
_________(2 sets of 23) in a regular cell
23 (one set) in a __________________
You inherit 1 set of chromosomes (23) from your mother and one set (23) from your father.
Your sex cells (sperm or egg) only have ______________ that is randomly made from a mixture of your two sets.
The Basics
Chromosomes are found in the _________________________
They typically take the form of _____________________
Chromosomes are made of_________ (deoxyribonucleic acid). DNA is in the shape of a spiral double helix
(twisted ladder)
DNA has three parts
Sugar (deoxyribose
_______________________
Nitrogen base (Adenine, Cytosine, Thymine, or ___________________)
The sugar and phosphates make up the sides of the ladder. The __________________ make up the rungs.
DNA
It is the sequence of ________________________ (A,C,T, G) that make each person’s DNA unique.
A is always found paired with T; _______________________________________________________
However, the large majority of the sequence is identical person to person (99.9%)
It is the _____________ difference that causes us to be unique.
There are about ________________________________ in a Human’s DNA.
The Basics
On those chromosomes there are about _________________________.
Genes are the basic unit of heredity.
Each gene has a single function (in other words it codes for a single protein.)
It codes for something by having a specific set of ______________________.
A change in the base pair order can change what the gene codes for.
Together, the proteins our genes code for make us who we are.
Alleles
Each person has _______________________ of each gene (one from each parent)
Those two copies are referred to as ____________________from that gene.
If the alleles are the same we say the person is____________________________
If the alleles are different we say the person is ____________________________
Tandem Repeats
A region of a chromosome that contains multiple copies of a DNA sequence arranged in a repeating fashion.
CAGCAGCAGCAGCAGCAGCAGCAGCAG
Roughly ___________________ of our DNA
Large variation in number of repeats from person to person. (This is what makes our DNA unique)
Follow basic Mendelian inheritance (Think Punnett Squares)
Genome
_______________________________________________________________________________
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DNA Fingerprinting  Scientists first discovered DNA in the 1800’s but it had no interest for the legal system until 1985 when___________________________ revealed his research that suggested that DNA is a unique as a fingerprinting. (thus the term DNA fingerprinting) The Basics  We have about 60 trillion cells and each of those cells contains a full set of our chromosomes  _________(2 sets of 23) in a regular cell  23 (one set) in a __________________  You inherit 1 set of chromosomes (23) from your mother and one set (23) from your father.  Your sex cells (sperm or egg) only have ______________ that is randomly made from a mixture of your two sets. The Basics  Chromosomes are found in the _________________________  They typically take the form of _____________________  Chromosomes are made of_________ (deoxyribonucleic acid). DNA is in the shape of a spiral double helix (twisted ladder)  DNA has three parts  Sugar (deoxyribose  _______________________  Nitrogen base (Adenine, Cytosine, Thymine, or ___________________)  The sugar and phosphates make up the sides of the ladder. The __________________ make up the rungs. DNA  It is the sequence of ________________________ (A,C,T, G) that make each person’s DNA unique.  A is always found paired with T; _______________________________________________________  However, the large majority of the sequence is identical person to person (99.9%)  It is the _____________ difference that causes us to be unique.  There are about ________________________________ in a Human’s DNA. The Basics  On those chromosomes there are about _________________________.  Genes are the basic unit of heredity.  Each gene has a single function (in other words it codes for a single protein.)  It codes for something by having a specific set of ______________________.  A change in the base pair order can change what the gene codes for.  Together, the proteins our genes code for make us who we are. Alleles  Each person has _______________________ of each gene (one from each parent)  Those two copies are referred to as ____________________from that gene.  If the alleles are the same we say the person is____________________________  If the alleles are different we say the person is ____________________________ Tandem Repeats  A region of a chromosome that contains multiple copies of a DNA sequence arranged in a repeating fashion.  CAGCAGCAGCAGCAGCAGCAGCAGCAG  Roughly ___________________ of our DNA  Large variation in number of repeats from person to person. (This is what makes our DNA unique)  Follow basic Mendelian inheritance (Think Punnett Squares) Genome  _______________________________________________________________________________

VNTR

 Variable Number of Tandem Repeats  This is why _______________________ works  Refers to the length of ________________________ in the DNA of individuals  The chances of anyone having the same results are less than one in a million. Other types of DNA  The DNA we are familiar with is nuclear DNA. It is our genetic material.  However, there is also mitochondrial DNA in your mitochondria.  Mitochondrial DNA comes only from your _____________________________.  Why?  Your mitochondrial DNA matches all your maternal relatives. Nuclear DNA vs. Mitochondrial DNA  Nuclear DNA is more useful in that it can be linked to a _____________________________.  _____________________ DNA however lasts longer (more stable)  There are thousands of mitochondria in a cell so there is a lot of mitochondrial DNA in each cell. DNA Replication  Our body has to copy DNA every time we need a new cell.  DNA replicates through a process called semiconservative replication. It follows complementary base pairing rules.  The primary enzyme responsible is ______________________________ Why is DNA useful?

Where can DNA be found?

Reference samples  Reference samples are taken from:

Collecting DNA  Care should be taken to avoid contamination  Disposable forceps, change gloves between items, Package separately  Must be air dried before packaging to avoid _____________ (which destroys the evidence)  Evidence should be kept __________________  Contamination is usually easily seen in the lab (if more than 2 bands are present)  If blood can’t be seen it can be detected with _________________ without any damage to the DNA. Collection of DNA  It is necessary only to collect 36 dna-bearing cells in order to have enough DNA to test and some new tests can go as low as 9 cells

 Because the strands are so short they can often be recovered from a body even after tremendous decomposition  Short length makes the ideal for PCR replication STR  Once copied the fragments can be run on an electrophoresis gel and their length can be determined (number of repeats)  Hundreds of STRs exist  _______________________have been used to form the national database (CODIS)  The more STRs that match the better the probability CODIS  _________________________________________________  All 50 states mandate the collection of DNA from criminals convicted of certain crimes (types of crimes are decided by states)  Sex offender  Felony offender  Felony arrest  All arrest (Virginia)  Maintained by the FBI but it contains databases from all jurisdictions.  Over 1.4 million profiles CODIS  Based on 13 STRs; when all 13 match a sample---it is considered a match  Functions:  Provides a way to link serial crimes  ______________________________________ Amelogenin gene  When conducting STR analysis, scientists look at the amelogenin gene to determine sex.  This gene is on both the X and Y chromosomes but it is _________________________________________  In a female you should only see _________________ on the gel whereas in a male you should see 2. Y-STRs  New test that looks only at STRs on the Y chromosome  Can help in sexual assault cases  Female DNA (victim) will not be present since she doesn’t possess the Y chromosome.  _____________________________________________________